MORPHOLOGICAL CHARACTERISTICS OF HEPATOTOXIC EFFECTS OF STABLE STRONTIUM CHLORIDE IN CHRONIC EXPERIMENTS IN RATS

Lugоvskyi S.P., Shlopov V.G., Sudya D.A. MORPHOLOGICAL CHARACTERISTICS OF HEPATOTOXIC EFFECTS OF STABLE STRONTIUM CHLORIDE IN CHRONIC EXPERIMENTS IN RATS. Український журнал з проблем медицини праці (2(43). с. 26-31. ISSN 2223-6757

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Background. In recent years, stable strontium and its compounds have found their wide application in various branches such as industry, agriculture, medicine, as well as in everyday life. However, aspects of the toxic effects of metal and its compounds on the body are not yet fully understood. Purpose of the study. To study hepatotoxicity effects of stable strontium chloride in chronic experiments in rats, according to morpho-functional data. Materials and methods. An experimental model of chronic strontium intoxication was used on male rats (n = 36), daily (5 days a week for 4 months) in intragastric gavages of an aqueous solution of strontium chloride in the stable dose of 1/30 DL 50 . The hepatotoxic evaluation of stable strontium chloride effects was made according to histological and histochemical study on rat liver after 1, 2, 3 and 4 months. For this purpose histological specimens were stained with hematoxylin & eosin, toluidine blue, ammoniac solution of silver nitrate, mixture of sudan III & IV. Histochemical reactions were carried out: PAS (perio dic acid–Schiff) in combination with diastase (PAS-D stain) for detection of glycogen, as well as the coupling with naphthol AS- BS- phosphate for detecting the activity of acid phosphatase (AP). Results. The studies have shown that chronic exposure to low doses of stable strontium chloride, are accompanied by development of hepatotoxic effects, which are characterized by: blood circulation impairment (blood stasis in hepatic veins and sinusoidal capillaries, especially in hepatic acini zone III); stromal edema and organ hyalinosis with collagen fibers; lipo- and protein dystrophy of hepatocytes, developed due to the reduced amount of glycogen in hepatocytes; hypertrophy and hyperplasia of Kupffer cells, as well as the increase in the amount of Kupffer cells in hepatocytes, due to histochemical activity of AP. Conclusion. Chronic exposures to stable strontium chloride in small doses, at 1/30 DL 50 causes development of drastic effects due to hepatotoxic metal action, which should be taken into consideration ,when assessing the risk of exposure to this metal both for industrial and household environment.

Тип елементу : Стаття
Додаткова інформація: УДК 546.42’131:616.36-099-092.9
Ключові слова: stable strontium, intoxication, experiment, hepatotoxic effects, стабильный стронций, интоксикация, эксперимент, гепатотоксическое действие, стабільний стронцій, інтоксикація, експеримент, гепатотоксична дія
Теми: 4. Медицина праці > 4.3. Гігієна праці
4. Медицина праці > 4.4. Теоретична медицина
4. Медицина праці > 4.9. Клінічні дослідження
Організація-джерело ресурсу: Вищі навчальні заклади
Наукові установи України > Національна академя медичних наук України
Назва організації: SI «Institute for Occupational Health of NAMS of Ukraine», Kiev, Donetsk National Medical University, Odessa National Medical University
Видавець: ТОВ «Видавничий дім «Авіцена»
Останні зміни: 27 Січ 2016 13:30
URI: http://opb.org.ua/id/eprint/3238

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